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Part b shows a synthetic mammalian genetic switch for tight, tunable and reversible control of a desired gene for therapeutic or genedelivery applications. In the OFF configuration (upper panel), expression of the gene of interest (green) is repressed at the levels of both transcription and translation. Constitutively expressed LacI repressor (red) binds to the lac operator sites in the transgene module of the gene of interest, therefore repressing its transcription.
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